Anti-PAK2 (Serine/Threonine-protein Kinase PAK 2, Gamma-PAK, PAK65, S6/H4 Kinase, p21-activated Kina

PAK2 is a widely expressed 58-62kD cytoplasmic ser/thr kinase of the PAK family, with substrates including ribosomal S6 and histone H4 proteins. The 524aa human PAK2 contains phosphorylation, acetylation (K38, K128) and myristoylation (G213) sites, a CRIB (cdc42/Rac interactive binding) domain aa74-87, a caspase-3 cleavage site after aa212, and a kinase domain aa249-499. Full-length PAK2 stimulates cell growth and motility, while the caspase cleaved PAK2 kinase domain 34kD is involved in cell death. Within the region used as an immunogen, human PAK2 shares 87% aa identity with mouse and rat PAK2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.