Anti-LGR5, loop2 (LGR5, GPR49, GPR67, Leucine-rich repeat-containing G-protein coupled receptor 5, G

LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:100, Flow Cytometry: 1:10-1:50, Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.