Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
I8427-03E.500 | 500 µg | - | - |
3 - 19 business days* |
1,189.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Interleukin 5 (IL-5) is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5... more
Product information "Anti-Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)"
Interleukin 5 (IL-5) is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry. Other applications have not been tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml. Detection limit foe rhIL5 is ~2ng/well., Western Blot: 1-2ug/ml. Detection limit foe rhIL5 is ~25ng/lane, Immunohistochemistry: 5-15ug/ml. Human peripheral blood lymphocytes or activated T-cells may be fixed for 20 minutes at RT with freshly prepared 1-2% paraformaldehyde/PBS (pH 7.4). Incubate cells for 60 minutes at RT with I8427-03E. , Flow Cytometry: 25ug/mL. For Flow Cytometry on PMA plus Ca2+ ionomycin-treated PBMCs, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS to detect intracellular staining of IL-5. Dilute I8427-03E to 25ug/mL and add 10ul of the diluted solution to 1-5x10e5 cells in a total reaction volume not exceeding 200ul. Visualize staining by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome*. , Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-65V IgG, F(ab')2 (FITC) Pab RbxMo, I1904-66A IgG, F(ab')2, X-Adsorbed (FITC) ) Pab RbxMo, , Storage and Stability:, Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.,
Keywords: | Anti-TRF, Anti-IL5, Anti-IL-5, Anti-Interleukin-5, Anti-T-cell replacing factor, Anti-B-cell differentiation factor I, Anti-Eosinophil differentiation factor |
Supplier: | United States Biological |
Supplier-Nr: | I8427-03E |
Properties
Application: | ELISA, FC, IHC, WB |
Antibody Type: | Monoclonal |
Clone: | 8H215 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | Sf 21-derived rhIL-5 |
Format: | Affinity Purified |
Database Information
KEGG ID : | K05428 | Matching products |
UniProt ID : | P05113 | Matching products |
Gene ID | GeneID 3567 | Matching products |
Handling & Safety
Storage: | +4°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
-30 %
Discount Promotion
-30 %
Discount Promotion
-30 %
Discount Promotion
-30 %
Discount Promotion
-30 %
Discount Promotion
Viewed