Anti-Integrin, alpha 1 (ITGA1, CD49a, CD49a Antigen, Laminin and Collagen Receptor, Very Late Activa

Integrin a1 is a 190-210kD member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin a1 forms a noncovalent heterodimer with Integrin b1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin a1 is a 1151aa type I transmembrane glycoprotein that contains a 1113aa extracellular domain (ECD) and a 15aa cytoplasmic tail. The ECD contains one vWFA/I-domain aa147-360 that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa765-1179. Over aa29-1141 (the ECD), human Integrin a1 shares 88aa identity with mouse Integrin a1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.