Anti-HIF2a

0.5mg/ml if reconstituted with 0.2ml sterile DI water. HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human. The human gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of HIF2a and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event. Moreover, the predicted protein shares 48% sequence identity with HIF1a, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1a, HIF2a binds to and activates transcription from the HIF1a response element derived from the 3-prime flanking region of the EPO gene. HIF2a is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, it may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia. It is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined. In addition, the variation in HIF-2 alpha influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration. Protein function: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. [The UniProt Consortium]