Anti-EIF4B (S422) (Eukaryotic Initiation Factor 4B, Eukaryotic Translation Initiation Factor 4B, hCG

EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F, Applications: Suitable for use in FLISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: FLISA: 1:1,000, Western Blot: 1:50-1:100, Immunohistochemistry: 1:10-1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap., , Note: Applications are based on unconjugated antibody.