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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
C8453-03.250 | 250 µl | - | - |
3 - 19 business days* |
298.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of... more
Product information "Anti-Cyclin B1"
In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates , Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B p34cdc2 plays a critical role in G2 to M transition. Applications: Suitable for use in ELISA and Western Blot. Other applications not determined. Recommended Dilutions: Western Blot: 2-4ug/ml for 2hrs at RT, Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells, Breast carcinoma. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.,
Supplier: | United States Biological |
Supplier-Nr: | C8453-03 |
Properties
Application: | ELISA, WB |
Antibody Type: | Polyclonal |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human, mouse, rat |
Immunogen: | synthetic peptide derived from C-terminus of human cyclin B1 protein. |
Format: | Affinity Purified |
Database Information
Handling & Safety
Storage: | +4°C (frozen cool packs!) |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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