Anti-CD80 (CD80 Antigen, CD80 Molecule, Activation B7-1 Antigen, B-lymphocyte Activation Antigen B7,

CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a single Ig-v-like domain, a single Ig-c-like domain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33kD, the presence of multiple (8) potential N-glycosylation sites results in a migration corresponding to ~60kD. Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 involved in T cell activation and proliferation. Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics. CD80 is expressed by activated B cells, and is important in B cell-T cell interactions, being a ligand of CD28 and CD152 (CTLA-4). CD80, also known as B7-1, a ~60kD type 1 trans-membrane protein expressed of macrophages, dendritic cells and activated B-cells. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells or 100ul whole blood, Western Blot: Non-reducing conditions, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.