Anti-CD80 (Biotin)

Human CD80 (B7-1) is a costimulatory ligand for CD28 and CTLA-4. CD80 is expressed on activated B cells. C2432-01F blocks binding of soluble CD152 Ig fusion protein to CD80. Applications: Suitable for use in Flow Cytometry and ELISA. , Other applications not tested. Recommended Dilution: Flow Cytometry: 80ul at 5ug/ml labels 5x10e5 cells., Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1.Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul C2432-01F at 5ug/ml. , 2. Cells were then washed twice, incubated with secondary reagent., 3. Cells were then washed three times, fixed and analyzed by FACS., 3. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. , 4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2432-01E. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.