Anti-CD35, C3b (CR1, Follicular Dendritic Cell Marker)

CD35 (CR1) is a type 1 membrane glycoprotein participating in clearance of immune complexes, regulation of autologous complement activation on cell surfaces, and complement- dependent cellular cytotoxicity mediated by leukocytes. CD35 inhibits complement activation by two distinct mechanisms. CD35 is a cofactor for factor I cleavage of C3b, iC3b and C4b. Furthermore CD35 mediates decay-dissociation of C3 and C5 convertases. CD35 is found on erythrocytes, neutrophils, monocytes and dendritic cells. There are four allotypic variants of CR35 (CR1) with different molecular masses: 190kD (CR-1A), 220kD (CR1-B), 160kD (CR1-C) and 250kD (CR1-D). , , Applications:, Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry (frozen and paraffin), Immunoassay, and Immunopurification. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10, Western Blot: 1:10, Immunohistochemistry: 1:10, Immunoprecipitation: 1:10, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.