Anti-Cathepsin D

Cathepsin D is an aspartyl protease expressed in all cells and has a role on the degradation of proteins in lysosomes. It is initially expressed as an inactive 52kD precursor and activated by proteolysis. This antibody detects both precursor and mature forms. It does not react with human cathepsins B, C, G and H and with bovine cathepsins D and B. Applications: Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml , Immunohistochemistry (FFPE): 1-2ug/ml, Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.