Anti-Cadherin E (Cadherin-1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Cadherins are a multifunctional family of Ca 2+ -dependent, transmembrane glycoproteins, which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous (reviewed in ref 1). Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue speci?c fashion. (2) Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. (3) In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and g-catenin which both bind to the actin binding protein a-catenin. Applications: Suitable for use in Inhibition of E-Cadherin dependent cell-cell contact and epitope mapping (10). Other applications have not been tested. Recommended Dilution: Inhibition of E-Cadherin dependent cell-cell contact: 200ug/ml for adhesion blockage., Optimal dilutions to be determined by researcher. Hybridoma: Myeloma cell line P3-X63-Ag8, Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile buffer containing 40-50% glycerol, dd2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.