Anti-Cadherin 5 (L-CAM, Epithelial Cell Adhesion Molecule, Vascular Endothelial, VE, CDH5, CD144)

Human vascular endothelium cadherin is an endothelium-specific cadherin localized at the intercellular junctions. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton. Applications: The antibody can be used for immunoassays, Western Blots, Immunoprecipitation and for purification. Furthermore the antibody is useful for Flow Cytometry and for inhibition in biological assays. Recommended Dilution: Immunohistochemistry: 1:10. Useful for staining of frozen sections., Flow Cytometry: 1:10, Western Blot: 1:10, Neutralization: For neutralization of biological activity in vitro dilutions have to be made according to the amounts of VE-cadherin to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.