These cookies are necessary for the basic functions of the shop.
Allow all cookies
Decline all cookies
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Conversion and usertracking via Google Tag Manager
Product information "Anti-His-Tag (6-Histidine), clone 3H2201"
The His-tagged expression system is widely used in the production of recombinant proteins. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by single-step chromatography. The His-tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500-1:1000, Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo, I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo, I1904-65C: IgG, H&L (HRP) Pab Rb x Mo, I1904-65H: IgG, H&L, X-Adsorbed (HRP) Pab Rb x Mo, I1904-65N: IgG (HRP) Pab Rb x Mo, I1903-07E: IgG (HRP) Pab Rb x Mo, I1904-67R: IgG H&L, X-adsorbed (HRP) Pab Rb x Mo, Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.