Our manufacturer AAT Bioquest, a specialist in fluorescence and luminescence technologies, offers an extensive range of kits for labeling antibodies and proteins as well as other biopolymers such as nucleic acids and carbohydrates. In this article, we would like to give you an overview of the leading product lines ReadiLink™, ReadiLink™ xtra and Buccutite™.
PCR is probably the most widely used molecular biological method today and an absolute standard tool for researchers. A further development of classical PCR is qPCR (real-time quantitative PCR), which in addition to amplifying DNA also allows quantification of the amplified genetic material.
Reactive oxygen species (ROS) are oxygen-containing molecules and are often simplified as "oxygen radicals". The study of ROS plays an important role in understanding cellular stress and various pathologies such as cancer, diabetes and cardiovascular diseases.
Gelite™ Safe DNA gel stain is an extremely sensitive and stable fluorescent dye for visualizing DNA in electrophoretic gels, with greater sensitivity and lower background fluorescence than both ethidium bromide (EtBr) and SYBR® Safe.
With so many possibilities, it can be difficult and tedious to select the appropriate calcium indicator for a given calcium ion flux assay. To streamline the process, here is what we consider to be the eight best single-wavelength, green fluorescent calcium indicators available.
Fluorescent indicators that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers.
AAT Bioquest offers a variety of dark-FRET peptide substrates and fluorogenic substrates which can be effectively used to screen for inhibitors of coronavirus proteases, papain-like proteases and more.
Fluorescence is the result of a three-stage process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes.
With over 50 different probes on the market, it can be a bit overwhelming to determine the optimal DNA dye for a given experiment. To simplify the process, here is what we consider the 10 best DNA dyes.