Hexokinase 4, Control Peptide (Glucokinase, Hexokinase Type IV, HK IV, Hexokinase-4, HK4, Hexokinase

Control peptide for H2040-51M. Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P. Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate. At least 4 related hexokinase isoforms (HXKI-III, HXK-IV also known as Glucokinase) have been cloned and characterized. Hexokinases (~100kD for HXK1-III, HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins. The N-terminus, containing the mitochondrial target sequence, and the C- terminal has high sequence homology among various isoforms. The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus. HXK4 (mouse 465aa, isoforms 1) isoforms are produced by use of alternative promoters. The use of alternative promoters apparently enables the type IV hexokinase gene to be regulated by insulin in the liver and glucose in the beta cell. This may constitute an important feedback loop for maintaining glucose homeostasis. HXK4 isoform 2 (missing 122-138aa) and HXK4-3 (isoforms 3) containes a different N- terminus from 1-15aa (MLDDRARMEATKKEK- >MAMDTTRCGAQLLTL). Alternative names for HXK4. EC 2.7.1.1, Hexokinase type IV, HK IV, HK4, Glucokinase (Gck). , Source:, Synthetic 19aa peptide sequence from mouse HXK4, C-terminal. Molecular Weight: ~3kD, Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.