Vascular Endothelial Growth Factor 165, Recombinant, Human (VEGF 165)

Description:, Recombinant human VEGF165 (GenBank Accession No. NM_001025368) is a 38.2kD disulfide-linked homodimeric protein (aa207-371), consisting of two 165 amino acid polypeptides. Source: Recombinant human VEGF165 expressed in E. coli. Amino Acide Sequence: ApmaegggqnhhevvkfmdvyqrsychpietlvdifqeypdeieyifkpscvplmrcggccndeglecvPteesnitmqimrikphqgqhigemsflqhnkcecrpkkdrarqenpcgpcserrkhlfvqdpqtckcsckntdsrckarqlelnertcrcdkprr, Biological Activity: ED50 is < ng/ml as determined by the dose-dependent stimulation, of the proliferation of human umbilical vein endothelial cells (HUVEC). Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.