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![R-Spondin 1 / RSPO1 Protein (His tag) (recombinant mouse)](/custom/plugins/NetiThemeBiomol/Resources/Themes/Frontend/Biomol/frontend/_public/src/img/no-picture.jpg)
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
E-PKSM040742.100 | 100 µg | - |
7 - 16 business days* |
895.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Activity: Measured by its ability to induce activation of ß-catenin response in a Topflash... more
Product information "R-Spondin 1 / RSPO1 Protein (His tag) (recombinant mouse)"
Activity: Measured by its ability to induce activation of ß-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a. Protein Construction: A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus. Sequence: Met 1-Gln 265. Fusion tag: C-His Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method. Apparent Molecular Mass: 44 kDa. Protein function: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6. Protein function: Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes (PubMed:21693646). Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1- mediated internalization of LRP6 through an interaction with KREM1. [The UniProt Consortium]
Keywords: | Rspo1, Cristin-3, mCristin-3, R-spondin-1, Roof plate-specific spondin-1, Cysteine-rich and single thrombospondin domain-containing protein 3, Recombinant Mouse R-Spondin 1 / RSPO1 Protein (His tag) |
Supplier: | Elabscience |
Supplier-Nr: | E-PKSM040742 |
Properties
Conjugate: | No |
Host: | Hamster cells |
Species reactivity: | mouse |
MW: | 28.5 kD |
Format: | Lyophilized |
Database Information
KEGG ID : | K19471 | Matching products |
UniProt ID : | Q9Z132 | Matching products |
Gene ID | GeneID 192199 | Matching products |
Handling & Safety
Storage: | -80°C |
Shipping: | 4°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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