PlGF-2, Recombinant, Human (Placenta Growth Factor-2, PGFL)

PLGF-2 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PLGF-2 is expressed in umbilical vein endothelial cells and placenta. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration. PlGF-2 also signals through Neuropilin (NP-1) and can bind with high affinity to heparin. Recombinant human PLGF-2 is a 34kD disulfide-linked homodimeric protein of two 150aa polypeptide chains. Source: Recombinant protein corresponding to human PLGF-2, expressed in E. coli. Biological Activity: Determined by its ability to chemoattract human monocytes of using a concentration range of 5-50ng/ml. Endotoxin: <0.1ng/ug (1EU/ug), AA Sequence: LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCCGDAVPRR, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.