Platelet Derived Growth Factor AA (PDGF-AA), human recombinant (rHuPDGF-AA)

Human PDGF-AA homodimer is produced by genetically engineered yeast, and purified by ionic exchange chromatography and reverse-phase HPLC. This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. Biological Activity: Human PDGF-AA homodimer is fully active as measured by the mitogenic assay involving the stimulation of 3H-thymidine incorporation into NIH-3T3 cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.