Interleukin 6, Recombinant, Chicken (IL-6, CHIL-6, Interferon beta 2, IFNB2)

Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine, produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells and plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses. Recombinant protein corresponding to chicken IL-6, his-tagged, expressed in E. coli. , Applications:, Suitable for use in ELISA, Functional Assays and In vitro Assays. Other applications not tested. , Recommended Dilution: Functional Assays: Induces corticosterone secretion in vivo. , In vitro Assays: Induces proliferation of the IL-6 dependant murine hybridoma cell line 7TD1 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.