Interleukin-3, active (IL3), Recombinant, Rhesus Macaque

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages., This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. Biological Activity: Assay #1: Fully biologically active when compared to standard. The ED50 as determined by the dose- dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0ng/ml, corresponding to a specific activity of ? 2x10e5 IU/mg. Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than , 0.05ng/ml, corresponding to a specific activity of > 2.0x10e7 IU/mg. Sequence: APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ, Molecular Weight: 14.0kD, PubMed ID: 2251147, 2257297, Gene Name: IL3, Swiss Prot: P25140, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.