Interleukin-1 Receptor Antagonist (IL-1RA), human recombinant (rHuIL-1RA)

One of the most potent pro-inflammatory mediators is the early-acting cytokine interleukin-1 (IL-1), whose actions are regulated by the structurally related IL-1 receptor antagonist (IL-1 RA). IL-1 RA is a competitive IL-1 inhibitor and a powerful anti-inflammatory agent. Also, Several autoimmune and inflammatory diseases have been associated with an allelic polymorphism of the IL-1 RA gene. Recombinant human IL-1 RA was expressed in E. coli and consists of 153 amino acids. AA Sequence: MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE, Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.