Interleukin-1 receptor antagonist, active (Il1rn), Recombinant, Mouse

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha- dependent proliferation of murine D10S cells is less than 50ng/ml, corresponding to a specific activity of ? 2.0x10e4 IU/mg in the presence of 50 pg/ml rHuIL-1alpha. Sequence: RPSGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNI KLEEKIDMVP IDLHSVFLGI HGGKLCLSCA KSGDDIKLQL EEVNITDLSK NKEEDKRFTF IRSEKGPTTS FESAACPGWF LCTTLEADRP VSLTNTPEEP LIVTKFYFQE DQ, Molecular Weight: 17.3kD, PubMed ID: 1828262, 1830498, 8003626, 9550387, 1828896, 1834470, Gene Name: Il1rn, Il-1ra, Swiss Prot: P25085, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.