Interferon gamma (IFNg), rat recombinant, CHO (rrIFNg)

This glycosylated interferon is constitutively produced by Chinese Hamster Ovary (CHO) cells transfected with the chromosomal gamma interferon gene of the rat. Purification is performed from serum-free culture medium by affinity chromatography using monoclonal antibodies to rat gamma interferon. This ultra pure product is intended for laboratory use in in vitro assay systems or in experimental animals. Biological activity has been demonstrated in both rat and murine systems. The major advantage of CHO-cell derived recombinant rat gamma interferon over natural rat gamma interferon, is the absence of contaminating lymphokines. This ensures that the effects observed in experimental results can be attributed exclusively to the rat gamma interferon used. One unit of Rat Gamma Interferon approximates the bioactivity contained in one unit of the rat gamma interferon standard prepared by the TNO laboratory. , , Source:, Rat, CHO-derived , , Recomended Dilution:, For dilutions use protein stabilized phosphate buffered saline, pH 8. (Do not use fresh serum). , , Storage and Stability:, Lyophilized powder may be stored at-20°C. Stable for 12 months at -20°C. Reconstitute with 1ml distilled or de-ionized water. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.