Insulin-Like Growth Factor I (IGF1), rat recombinant (rrIGF1)

A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. Recombinant Rat IGF-1 is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7687 Dalton. , Biological Activity:, Rat IGF-1 is fully biologically active when compared to standards. The ED50, was calculated using 2 methods: , 1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be less then 30ng/ml. , 2. Type 1 IGF receptor binding assay ED50 was found to be less then 10ng/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.