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NPPB is a chloride channel blocker (IC50 = 80 nM) that has also been identified as a GPR35 agonist. It has been shown to activate the GPR35-Galphai/o and GPR35-Galpha16 pathways in HEK293 cells, inducing intracellular calcium mobilization. NPPB has protonophoric activity and has been used to uncouple mitochondrial ATP synthesis in phagocytes.Formal Name: 5-nitro-2-[(3-phenylpropyl)amino]-benzoic acid. CAS Number: 107254-86-4. Synonyms: HOE 144, Hoechst 144. Molecular Formula: C16H16N2O4. Formula Weight: 300.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 50 mg/ml, DMSO: 50 mg/ml, DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml, Ethanol: 1 mg/ml. lambdamax: 215, 366 nm. SMILES: OC(C1=C(NCCCC2=CC=CC=C2)C=CC([N+]([O-])=O)=C1)=O. InChi Code: InChI=1S/C16H16N2O4/c19-16(20)14-11-13(18(21)22)8-9-15(14)17-10-4-7-12-5-2-1-3-6-12/h1-3,5-6,8-9,11,17H,4,7,10H2,(H,19,20). InChi Key: WBSMIPAMAXNXFS-UHFFFAOYSA-N.
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