PGR Reporter Assay System (pig)

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Pig Progesterone Receptor (pgPGR). INDIGO's Pig PGR reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the pgPGR. In addition to Pig PGR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against Pig PGR. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture. Features Ready to Use Upon Receipt Includes All Needed Components Contains Transfected Reporter Cells Eliminates Cell Licensing Fees Clear, Reproducible Results Consistent Results Lot to Lot Product Specifications Target Type Nuclear Hormone Receptor, Nuclear Receptor Orthologs Species Pig Receptor Form Hybrid Assay Mode Agonist, Antagonist Kit Components Pig PGR Reporter Cells Cell Recovery Medium (CRM) Compound Screening Medium (CSM) Progesterone, 3 uM (in DMSO) Detection Substrate Detection Buffer White, sterile, cell-culture ready assay plate Shelf Life 6 months Orthologs Available Yes. Target Background: This nuclear receptor assay utilizes proprietary cells engineered to provide constitutive, high-level expression of the full-length Pig Progesterone Receptor (NR3C3), a ligand-dependent transcription factor commonly referred to as PR or PGR. INDIGO's Reporter Cells include the luciferase reporter gene functionally linked to a PGR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in PGR activity. Luciferase gene expression occurs after ligand-bound PGR undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the target gene. Unlike in vitro binding assays, and some other cell-based assay strategies, the readout from INDIGO's reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo. The principal application of this assay is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the pig progesterone receptor. It is an all-inclusive assay system that includes, in addition to PGR Reporter Cells, two optimized media for use during cell culture and for preparing dilutions of test samples, the reference agonist Progesterone, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol. Protein function: Steroid hormone receptor involved in the regulation of eukaryotic gene expression which affects cellular proliferation and differentiation in target tissues. [The UniProt Consortium]