INDIGlo Luciferase Detection Reagent Kit

INDIGlo Luciferase Detection Reagent (LDR) is formulated to deliver steady, long-lived luminescence signal from mammalian reporter cells expressing firefly luciferase. For convenience, the reagent is provided 'ready to use', without the requirement to first re-constitute powder-form components. INDIGlo LDR works equally well in quantifying expressed luciferase activity from 96- or 384-well format reporter assays. The resulting light emission is stable over a 2 hr period, thereby making INDIGlo LDR an ideal, economical detection reagent for both low- and high-throughput batch processing of assay plates. INDIGlo LDR is formulated as a 2x-concentrated reagent, thereby allowing greater versatility in its use. The 2x-concentrated reagent will be used when using 384-well or 1536-well assay plates that are processed using a homogenous assay strategy. Alternatively, when processing 96-well assay plates either a homogenous or non-homogenous assay strategy may be used. For convenience, this kit provides an optimized Dilution Buffer for use in diluting INDIGlo LDR when performing non-homogenous assays. Regardless of one's assay setup preference INDIGlo LDR delivers rapid lysis of the reporter cells, robust luminescence signal, and sensitive quantification of expressed luciferase activities.