GR Reporter Assay System (dog)

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Dog Glucocorticoid Receptor (dGR). INDIGO's dGR reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the Dog GR. In addition to dGR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against Dog GR. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture. Features Ready to Use Upon Receipt Includes All Needed Components Contains Transfected Reporter Cells Eliminates Cell Licensing Fees Clear, Reproducible Results Consistent Results Lot to Lot Product Specifications Target Type Nuclear Hormone Receptor, Nuclear Receptor Orthologs Species Dog Receptor Form Hybrid Assay Mode Agonist, Antagonist Kit Components Dog GR Reporter Cells Cell Recovery Medium (CRM) Compound Screening Medium (CSM) Dexamethasone, 3.0 uM (in DMSO) Detection Substrate Detection Buffer White, sterile, cell-culture ready assay plate Shelf Life 6 months Orthologs Available Yes. Target Background: This assay utilizes proprietary cells engineered to provide constitutive high-level expression of the native full-length Dog Glucocorticoid Receptor (NR3C1), a ligand-dependent transcription factor, commonly referred to as GR. INDIGO's Reporter Cells include the luciferase reporter gene functionally linked to a GR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in GR activity. Luciferase gene expression occurs after ligand-bound GR undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from INDIGO's reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo. INDIGO Bioscience's nuclear receptor assays are all-inclusive cell-based assay systems. In addition to Dog GR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, the reference agonist Dexamethasone, Luciferase Detection Reagent, and a cell culture-ready assay plate.