Reporter Assays

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human constitutive androstane receptor, isoform 3 (NR1I3), a ligand-dependent transcription factor commonly referred to as CAR3. These reporter cells utilize a modified...

This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the full-length human progesterone receptor (NR3C3), a ligand-dependent transcription factor commonly referred to as PR. INDIGO's reporter cells include the luciferase reporter gene...

This nuclear receptor assay system utilizes proprietary human mammalian cells engineered to provide high-level expression of a hybrid form of the human estrogen-related receptor alpha (NR3B1). The N-terminal DNA binding domains (DBD) of the native ERRalpha has been substituted with that of the yeast GAL4-DBD. The...

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide high-level expression of a hybrid form of the Human Estrogen-Related Receptor gamma (NR3B3). The N-terminal DNA binding domains (DBD) of the native ERRgamma has been substituted with that of the yeast GAL4-DBD....

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the mouse peroxisome proliferator-activated receptor delta (NR1C2), a ligand-dependent transcription factor commonly referred to as PPARD or PPARdelta. INDIGO's reporter cells include...

Protein function: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. [The...

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human liver X receptor beta (NR1H2), a ligand-dependent transcription factor commonly referred to as LXRbeta. INDIGO's reporter cells include the luciferase reporter gene...

This nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human thyroid hormone receptor alpha (NR1A1), a ligand-dependent transcription factor commonly referred to as TRalpha. INDIGO's reporter cells include the luciferase reporter gene...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the rat peroxisome proliferator-activated receptor alpha (NR1C1), a ligand-dependent transcription factor commonly referred to as PPARA or rPPARalpha. INDIGO's reporter cells include...

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The most versatile reporter gene is the firefly luciferase. Recently there is steadily increasing use of other luciferases, such as Renilla luciferase since these reporters are smaller and do not require the presence of ATP. Our...