Reporter Assays

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human liver X receptor alpha (NR1H3), a ligand-dependent transcription factor commonly referred to as LXRalpha. INDIGO's reporter cells include the luciferase reporter gene...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length human estrogen receptor 1 (NR3A1), a ligand-dependent transcription factor commonly referred to as ERalpha. INDIGO's reporter cells include the luciferase reporter gene...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive high-level expression of full-length, unmodified human glucocorticoid receptor (NR3C1), a ligand-dependent transcription factor, commonly referred to as GR. INDIGO's reporter cells include the luciferase...

Protein function: Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and...

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase. The advantages of a luciferase assay are the high sensitivity, the absence of luciferase activity inside most of the cell types, the wide dynamic range, rapidity and low cost. The most versatile and common reporter gene is the...

This nuclear receptor assay system utilizes a proprietary rodent cell line that is further engineered to express the mouse/rat peroxisome proliferator-activated receptor gamma (NR1C3, PPARG). Because the receptor's ligand binding domain sequence is conserved between mouse and rat species, the receptor is denoted...

Protein function: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin,...

This is an all-inclusive cell-based luciferase reporter assay kit targeting the the Human Adrenoreceptor Beta 2 (ADRB2). INDIGO's ADRB1 reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the ADRB2. In addition to ADRB2 Reporter Cells, this kit provides...

The CSF1R SRE Reporter Kit is designed for monitoring the activity of the CSF1R signaling pathway in cultured cells. The kit contains a transfection-ready vector for CSF1R and SRE luciferase reporter vector. Upon ligand binding, active CSF1R will initiate the MAPK/ERK signaling pathway, leading to expression of the...

The EP2 receptor is one of four GPCRs that mediate the actions of PGE2. The diverse effects of PGE2 acting via EP2 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP2...

Early screening of drug candidate's potential to induce or inhibit CYP2C9 is a critical step in the drug development process. The CYP2C9 Induction Reporter Assay Kit is a novel method to evaluate CYP2C9 induction by drug candidates. Cayman's CYP2C9 Induction Reporter Assay Kit consists of a 96-well plate coated with...

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human peroxisome proliferator-activated receptor gamma (NR1C3), a ligand-dependent transcription factor commonly referred to as PPARG or PPARgamma. INDIGO's reporter cells include...