Screen Quest(TM) Live Cell Opioid-Like receptor hMOR1 cAMP Assay Service Pack

hMOR1, also known as the Human Mu Opioid Receptor (MOR1), is a key member of the G-protein coupled receptor (GPCR) family. hMOR1 is a critical target for opioid-based analgesics and is implicated in various disorders, including chronic pain, opioid addiction, and other neurological conditions. Traditional methods for studying GPCRs may not fully capture the broad spectrum of signaling mechanisms that occur through specific receptors like hMOR1, which couples to both cAMP production and inhibition. The Screen Quest(TM) Live Cell Opioid-Like Receptor hMOR1 cAMP Assay Service Pack is designed for real-time, high-throughput monitoring of intracellular cAMP changes associated with hMOR1 activation using transfected cell lines and Calbryte(TM) 520 wash-free calcium fluorescence detection methods. Unlike conventional assays that require cell lysis, this assay preserves cellular integrity, enabling both temporal and spatial resolution of specific signaling events associated with hMOR1. This assay employs cell lines transfected to express hMOR1 alongside a promiscuous G-protein Galpha16. The co-expression of Galpha16 enables hMOR1, which normally signals via Gi/o-mediated inhibition of adenylyl cyclase and reduction in intracellular cAMP, to couple to Gq signaling, leading to calcium flux. Activation of hMOR1 by specific ligands such as µ-opioid peptide (endorphins) can be detected using calcium-sensitive dyes such as Calbryte(TM) 520 AM, Cal-520(R) AM, Fluo-8(R) AM, Fluo-4(TM) AM, or other corresponding no-wash calcium kits. This service pack provides all necessary components for precise measurement of hMOR1-mediated cAMP changes using FLIPR, FDSS, or equivalent fluorescence microplate readers. It enables researchers to explore hMOR1 signaling pathways and evaluate potential therapeutic compounds targeting this receptor, particularly for pain management and addiction-related research.