Screen Quest(TM) Live Cell Chemokine (CC) receptor CCR2B cAMP Assay Service Pack

CCR2B or Chemokine (CC) receptor type 2B, a key member of the GPCR family, is implicated in a variety of inflammatory, autoimmune diseases, cancer, fibrosis, and other pathological conditions, making it a vital therapeutic target. Traditional methods for studying GPCRs often fail to address the signaling mechanisms of specific GPCRs like CCR2B, which couple to adenylyl cyclase activity and intracellular cAMP production. The Screen Quest(TM) Live Cell Chemokine (CC) Receptor CCR2B cAMP Assay Service Pack is specifically designed for real-time, high-throughput monitoring of intracellular cAMP changes associated with CCR2B activation using transfected cell lines and Calbryte(TM) 520 wash-free calcium fluorescence detection methods. Unlike conventional assays requiring cell lysis, this assay preserves cellular integrity, enabling both temporal and spatial resolution of specific signaling events associated with CCR2B. This assay employs cell lines transfected to express CCR2B along with a promiscuous G-protein Galpha16. The Galpha16 protein allows CCR2B, which primarily signals through the cAMP pathway, to also couple to Gq signal transduction and mobilize intracellular calcium. Activation of CCR2B by specific ligands, such as CCL2 (MCP-1), can be detected using calcium-sensitive dyes like Calbryte(TM) 520 AM, Cal-520(R) AM, Fluo-8(R) AM, Fluo-4(TM) AM, or corresponding no-wash calcium kits. The inclusion of CCR2B and Galpha16 co-expression ensures robust calcium signaling for reliable assay performance. This service pack provides all necessary components for precise measurement of CCR2B-mediated cAMP changes using FLIPR, FDSS, or equivalent fluorescence microplate readers. It is an ideal tool for studying CCR2B activity, enabling researchers to explore CCR2B signaling pathways and evaluate potential therapeutic compounds targeting this receptor.