Screen Quest(TM) Live Cell Chemokine (C-X-C motif) receptor CXCR2 cAMP Assay Service Pack

CXCR2 or C-X-C motif chemokine receptor 2 belongs to the GPCR family, and plays a pivotal role in immune responses by mediating the effects of various chemokines. CXCR2 is implicated in various inflammatory, immune, and pathological conditions, including neutrophil recruitment, cancer progression, and angiogenesis, making it an important therapeutic target. However, traditional methods for studying GPCRs often fail to adequately capture the full spectrum of signaling mechanisms of specific receptors like CXCR2, which primarily couples to adenylyl cyclase and intracellular cAMP production. The Screen Quest(TM) Live Cell Chemokine (C-X-C motif) Receptor CXCR2 cAMP Assay Service Pack is specifically designed for real-time, high-throughput monitoring of intracellular cAMP changes associated with CXCR2 activation using transfected cell lines and Calbryte(TM) 520 wash-free calcium fluorescence detection methods. Unlike conventional assays that require cell lysis, this assay preserves cellular integrity, enabling both temporal and spatial resolution of specific signaling events associated with CXCR2. This assay employs cell lines transfected to express CXCR2 along with a promiscuous G-protein Galpha16. The Galpha16 protein allows CXCR2, which primarily signals through the cAMP pathway, to also couple to Gq signal transduction and mobilize intracellular calcium. Activation of CXCR2 by specific ligands, such as IL-8 or Gro-alpha, can be detected using calcium-sensitive dyes like Calbryte(TM) 520 AM, Cal-520(R) AM, Fluo-8(R) AM, Fluo-4(TM) AM, or corresponding no-wash calcium kits. The inclusion of CXCR2 and Galpha16 co-expression ensures robust calcium signaling for reliable assay performance. This service pack provides all necessary components for precise measurement of CXCR2-mediated cAMP changes using FLIPR, FDSS, or equivalent fluorescence microplate readers. It is an ideal tool for studying non-Gq protein-coupled CXCR2 activity, enabling researchers to explore CXCR2 signaling pathways and evaluate potential therapeutic compounds targeting this receptor.