S-Glutathionylated Protein Detection Kit

S-Glutathionylated Protein Detection Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay10010721-1 1 each -

6 - 10 business days*

Glutathione (GSH) is a tripeptide (gamma-glutamylcysteinylglycine) widely distributed in both... more
Product information "S-Glutathionylated Protein Detection Kit"
Glutathione (GSH) is a tripeptide (gamma-glutamylcysteinylglycine) widely distributed in both plants and animals. GSH is involved in maintenance of protein sulfhydryl reduction status. The concentration of GSH ranges from a few micromolar in plasma to several millimolar in tissues such as liver. Mixed protein glutathionyl disulfides are a post translational protein modification of growing interest. Protein S-glutathionylation (PSSG) may modify the activity of a large number of cell proteins, including metabolic, structural, cytoskeletal, and signaling proteins. PSSG detection methods can employ GSH adduct antibodies, GSH derivatives, and differential labeling systems based on the "Biotin-Switch" method. Cayman's S-Glutathionylated Protein Detection Assay Kit provides a convenient method for the direct visualization of S-glutathionylated proteins in whole (permeabilized) cells by flow cytometry and microscopy as well as avidin overlay analysis. This cell-based assay starts with the modification of protein free-thiols groups followed by enzymatic cleavage of any protein-S-glutathione (PSSG) adducts present in the sample. Biotinylation of the newly-formed protein free-thiols provides the basis for visualization using streptavidin-based colorimetric or fluorescence detection. Reagents are provided to test three sets of 10 samples (most convenient) or up to thirty samples total at once if desired. Synonyms: PSSG. Origin: Plant/Armoracia rusticana.
Keywords: PSSG
Supplier: Cayman Chemical
Supplier-Nr: 10010721


Application: S-Glutathionylated Protein Detection
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H302, H311, H314, H315, H316, H317, H318, H319, H332, H335
P Phrases: P260, P261, P264, P270, P271, P272, P280, P301+310, P301+330+331, P302+352, P303+361+353, P304+340, P305+351+338, P310, P312, P321, P330, P332+313, P333+313, P337+313, P361+364, P362+364, P363, P403+233, P405, P501
Our products are for laboratory research use only: Not for administration to humans!
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