Extracellular Oxygen Consumption Assay Kit

Cayman's Extracellular Oxygen Consumption Assay Kit uses a phosphorescent oxygen-sensitive probe. Because of its inability to cross the membrane, the probe selectively reports on changes in oxygen levels in the medium. The probe's signal is quenched upon binding to oxygen, resulting in a signal that is inversely proportional to the amount of dissolved oxygen present in culture medium. As mitochondria consume oxygen during respiration, the extracellular oxygen concentration decreases, leading to an increase in signal intensity over time. This assay is compatible with standard fluorescence plate readers and provides a simple and sensitive method for determining OCR in living cells and isolated mitochondria.