Cellular Cuprous Fluorometric Assay Kit

Detection principle: Abnormal accumulation of cellular cuprous in cells induces the oligomerization of key components of the lipoic acid-modified pyruvate dehydrogenase complex, which affects the tricarboxylic acid cycle, triggers proteotoxic stress, and induces cell death. Cellular cuprous can catalyze the substrate to produce fluorescent substances, and the higher the copper ion concentration, the more fluorescent substances are generated per unit time. The fluorescence delete can be detected at the excitation wavelength of 395 nm and emission wavelength of 480 nm. Performance characteristics: Sample type: cell. Detection method: Fluorescence method. Assay type: Quantitative. Assay time: 50 min. Precision Average inter-assay CV: 5.0-8.0%. Average intra-assay CV: 3.0-6.0%. Other reagents required: Normal saline (0.9% NaCl), Double distilled water. Storage -20?. Valid period 12 months.