Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit

Detection principle: Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The activity of mitochondrial complex I can be quantified by measure the change OD value at 340 nm. Performance characteristics: Synonyms: NADH-CoQ Reductase, Sample type: cell, Sensitivity: 2.85 U/L, Detection range: 2.85-119.04 U/L, Detection method: Colorimetric method, Assay type: Enzyme Activity, Assay time: 30 min. Precision: Average inter-assay CV: 5.0-8.0%, Average intra-assay CV: 3.0-4.0%. Other reagents required: Anhydrous ethanol (AR). Storage: -20?. Valid period: 12 months. Protein function: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:14729820, PubMed:30140060). Essential for the catalytic activity and assembly of complex I (PubMed:14729820, PubMed:24028823, PubMed:30140060). [The UniProt Consortium]