Other Cell-Based Assays

Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative...

Quantitate and monitor intracellular cathepsin K activity over time in vitro. The Magic Red reagent in this assay fluoresces red upon cleavage by active cathepsin enzymes. Analyze the fluorescent signal using fluorescence microscopy or a fluorescent plate reader. This kit is based on a cell-permeant fluorescent...

The kit uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably vial the lysosome pH gradient. The dye easily permeates intact live cells, and trapped in lysosomes after it gets into cells.

In mammals, triglycerides are constantly synthesized from fatty acids and segregated into cytosolic lipid droplets, mainly in adipocytes, as the major energy storage depot. During fasting, triglycerides stored in adipose tissue and liver are hydrolyzed by hormone-sensitive lipase and adipose triglyceride lipase to...

As one of the major sources of reactive oxygen species (ROS) in the cell, mitochondrial ROS has often been difficult to single out from the other sources of ROS within the cell. While some ROS dyes such as DHE and DCFDA tend to be less location-specific and dyes such as ADHP are only extracellular, Mitochondrial ROS...

Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative...

This particular kit is designed to uniformly label live cells in red fluorescence for the studies that require the fluorescent tag molecules retained inside cells for relatively longer time.

Glutathione (GSH) is a tripeptide (gamma-glutamylcysteinylglycine) widely distributed in both plants and animals. GSH is involved in maintenance of protein sulfhydryl reduction status. The concentration of GSH ranges from a few micromolar in plasma to several millimolar in tissues such as liver. Mixed protein...

Low-density lipoproteins (LDL) is the major carrier of cholesterol in the blood, accounting for more than 60% of total plasma cholesterol. LDL is taken up by hepatic and extrahepatic tissues through LDL receptor-mediated endocytosis where it is internalized and degraded to free cholesterol and amino acids. Uptake of...

This kit is based on a cell-permeant fluorescent probe (MR-(FR)2) designed for real-time detection of cathepsin L activity within whole living cells, using a fluorescence microscope. Excitation wavelength 560nm, emission 610-650nm.

Detection Range: 0.19-100.00 nmol/mL. Test Principle: Colorimetric method. Sensitivity: 0.19 nmol/mL

The kit uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably vial the lysosome pH gradient. The dye easily permeates intact live cells, and trapped in lysosomes after it gets into cells.