Other Cell-Based Assays

The CTC Flow Cytometric Live Bacteria Assay Kit provides an easy and convenient method for evaluating bacterial health and vitality as a function of the respiratory activity. CTC itself is non-fluorescent, once reduced by the electron transfer system of viable bacterial cell surface, red and insoluble fluorescent...

Cell cycles have four sequential phases: G0/G1, S, G2, and M. During a cell's passage through cell cycle, its DNA is duplicated in S (synthesis) phase and distributed equally between two daughter cells in M (mitosis) phase. These two phases are separated by two gap phases: G0/G1 and G2. The two gap phases provide...

Our Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of MMP coincides with the opening of the...

The materials provided in the assay kit for MDR1 / Human P-Glycoprotein Drug Interaction are formatted to allow for two independent 48-well assay setups. Each aliquot of HCT-Pgp Cells is provided as a single-use assay reagent and provides sufficient volume to dispense 48 assay wells of HCT-Pgp cells into one 96-well...

The reagents and materials provided in the assay kit for the Expression Profiling of Clinically Relevant CYPs are formatted to allow for two alternative cell culture setups. In one scenario 48 culture wells may be set up at two different times. In the other assay scenario 96 culture wells may be set up at one time....

The principal application of this assay is to rapidly screen test compounds to identify those that induce acute liver cell toxicity. This kit includes two aliquots of cryopreserved Luminescent Reporter Hepatocytes (upcyte(R)), donor 10-13, isolated from an adult Caucasian female, that have been further modified to...

Green Cathepsin B Assay allows researchers to detect and monitor intracellular cathepsin activity over time in vitro using live whole cells. This in vitro assay employs the quenched substrate Rhodamine 110-(RR)2, which fluoresces green upon cleavage by active cathepsin enzymes. Analyze samples using flow cytometry...

Assess changes in intracellular glutathione levels with the Intracellular GSH Assay Kit. This whole cell glutathione assay employs a proprietary thiol-sensitive dye, ThioBright(TM) Green, to monitor relative changes in the concentration of the free form of glutathione, aka GSH. Analyze the fluorescent signal by flow...

Intracellular Total ROS Activity Assay provides a good screening option for assessing the potency of oxidative stress inhibitor and activator reagents, and will help to determine how oxidative stress modulates varied intracellular pathways. This product assesses the overall level of intracellular ROS activity and...

Nitric Oxide Synthase Assay provides a good screening option for assessing the potency of nitrosative stress inhibitor and activator reagents, and will help to determine how oxidative and nitrosative stress modulates varied intracellular pathways. This kit assesses the overall intracellular levels of free nitric...

Excitation (nm): 490. Emission (nm): 525. Quantum yield: 0.95. G protein coupled receptors (GPCR) are one of the largest receptor classes targeted by drug discovery programs. Calcium flux (coupled via Gq pathway) assay is a preferred method in drug discovery for screening GPCR targets. However, over 60% of the known...