Other Cell-Based Assays

ICT's Nitric Oxide Synthase Assay provides a good screening option for assessing the potency of nitrosative stress inhibitor and activator reagents, and will help to determine how oxidative and nitrosative stress modulates varied intracellular pathways. This kit assesses the overall intracellular levels of free...

This MPO / Myeloperoxidase Activity Assay Kit (Colorimetric) can be used to measure Myeloperoxidase activity in whole neutrophils, neutrophil lysates, tissue homogenates and plasma (EDTA). Please note that this kit does not include a microplate. Protein function: Part of the host defense system of polymorphonuclear...

Extinction coefficient (cm^-1 M^-1): 250000 Excitation (nm): 651 Emission (nm): 670 Quantum yield: 0.27 (PBS), 0.4 (ethanol). Glucose metabolism is a primary source of cellular energy and biomaterials for maintaining cell homeostasis. Surplus glucose is stored as glycogen in muscular and hepatic tissues, with...

Detection principle: Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The activity of mitochondrial complex I can be quantified by measure the change OD value at 340 nm. Performance characteristics: Synonyms: NADH-CoQ Reductase, Sample type:...

Detection principle: Mitochondrial complex II, also known as succinate-coenzyme Q reductase, is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. It catalyzes the oxidation of succinic acid in the TCA cycle to fumaric acid, which converts the ubiquinone to a reduced form through...

Detection principle: Mitochondrial complex III, also known as cytochrome c reductase complex, its main function is to oxidize the reduced coenzyme Q10 formed by mitochondrial complexes I and II to oxidative coenzyme Q10. In this process, the OD value increased at 550 nm. Therefore, the activity of mitochondrial...

Detection principle: Mitochondrial complex IV, also known as cytochrome C oxidase, is one of the major enzymes in the mitochondrial respiratory chain. It oxidizes the reduced cytochrome C converted from mitochondrial complex III to oxidized cytochrome C and consumes oxygen to generate water. Mitochondrial complex IV...

Test Principle: Fluorometric method.