ROS Detection Cell-Based Assay Kit (DHE)

ROS Detection Cell-Based Assay Kit (DHE)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay601290-96 96 wells -

6 - 10 business days*

207.00€
 
Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive... more
Product information "ROS Detection Cell-Based Assay Kit (DHE)"
Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of reactive oxygen species (ROS) to form ethidium (E+) (ex 480 nm/em 576 nm). Given their narrow spectral range, distinguishing between the two species using filter-based optical systems is often difficult and can lead to misreporting of the species of ROS being generated. This assay kit uses DHE as a fluorescent probe for the detection of ROS generation. Antimycin A, an inhibitor of complex III of the mitochondrial electron transport chain, is included as a positive control for ROS generation. N-acetyl Cysteine is included as an antioxidant control. Synonyms: Reactive Oxidation Species CBA Kit (dihydroethidium).
Keywords: Reactive Oxidation Species CBA Kit (dihydroethidium)
Supplier: Cayman Chemical
Supplier-Nr: 601290

Properties

Application: Cell based assays, FC, HTS

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H225, H227, H302, H402
P Phrases: P210, P233, P240, P241, P242, P243, P264, P270, P273, P280, P301+310, P303+361+353, P330, P370+378, P403, P403+235, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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