Cell Biology Assays

Lysosomes are intracellular organelles that contain enzymes used for the hydrolysis of waste materials and other cellular debris. The pH of the lysosome is ~4.5-4.8 and is optimal for these hydrolytic enzymes. The lysosomal pH gradient is maintained through vacuolar ATPases which pump protons into the lysosomes....

ICT's Autophagy Assay, Red enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer. Autophagy is a...

Detection principle: CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green...

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. Senescence-associated beta-galactosidase is highly overexpressed in senescent cells and has been widely used as a senescence marker. The colorimetric X-gal staining method is widely used to detect...

Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS). This process can lead to not only disruption of the cellular membrane integrity, but also inactivation of membrane-bound receptors. It is one of the main causes of free radical-mediated damages in cells. Cell...

Autophagy Assay, Red enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer.

Cell cycle refers to the whole process from the end of one mitosis to the end of the next. During this process, the genetic material is replicated and doubled, and evenly distributed to two daughter cells at the end of division. Cell cycle can also be divided into phases like interphase and Metaphase. Intercellular...

Cell cycle refers to the whole process from the end of one mitosis to the end of the next. During this process, the genetic material is replicated and doubled, and evenly distributed to two daughter cells at the end of division. Cell cycle can also be divided into phases like interphase and Metaphase. Intercellular...