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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
ABD-20100 | 25 tests | - |
3 - 8 business days* |
418.00€
|
Our Cell Meter(TM) live cell caspases activity assay kits are based on fluorescent FMK inhibitors... more
Product information "Cell Meter(TM) Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*"
Our Cell Meter(TM) live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. The activation of caspase 3/7 is important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses FAM-DEVD-FMK as a fluorescent indicator for caspase 3/7 activity. FAM-DEVD-FMK irreversibly binds to activated caspase 3/7 in apoptotic cells. Once bound to caspase 3/7, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 3/7 but will not stop apoptosis from proceeding. There are a variety of parameters that can be used for monitoring cell apoptosis. This Cell Meter(TM) Live Cell Caspase 3/7 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 3/7 activation in live cells. It is used for the quantification of activated caspase 3/7 activities in apoptotic cells, or for screening caspase 3/7 inhibitors. FAM-DEVD-FMK, the green label reagent, allows for direct detection of activated caspase 3/7 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.
Supplier: | AAT Bioquest |
Supplier-Nr: | 20100 |
Properties
Application: | Caspase 3/7 binding assay, FC |
Format: | Homog. |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +20°C (International: +20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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