Cell Meter(TM) Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*

Cell Meter(TM) Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-20100 25 tests -

3 - 8 business days*

346.00€
 
Our Cell Meter(TM) live cell caspases activity assay kits are based on fluorescent FMK inhibitors... more
Product information "Cell Meter(TM) Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*"
Our Cell Meter(TM) live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. The activation of caspase 3/7 is important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses FAM-DEVD-FMK as a fluorescent indicator for caspase 3/7 activity. FAM-DEVD-FMK irreversibly binds to activated caspase 3/7 in apoptotic cells. Once bound to caspase 3/7, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 3/7 but will not stop apoptosis from proceeding. There are a variety of parameters that can be used for monitoring cell apoptosis. This Cell Meter(TM) Live Cell Caspase 3/7 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 3/7 activation in live cells. It is used for the quantification of activated caspase 3/7 activities in apoptotic cells, or for screening caspase 3/7 inhibitors. FAM-DEVD-FMK, the green label reagent, allows for direct detection of activated caspase 3/7 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.
Supplier: AAT Bioquest
Supplier-Nr: 20100

Properties

Application: Caspase 3/7 binding assay, FC
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +20°C (International: +20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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