Apoptosis and Cell Cycle Assays

DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL).

The Caspase 9 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 9 recognition sequence (LEHD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has...

Cell Meter(TM) TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation. The assay is non-radioactive and rapid. The TUNEL assay uses the terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of TMR-dUTP at the free 3'-hydroxyl ends of the...

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. Senescence-associated beta-galactosidase (SA-beta-gal) is highly overexpressed in senescent cells and it has been widely used as a senescence marker. X-gal staining, a colorimetric method is widely...

Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Labeld Annexin V bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be used for detection of apoptosis.

Elabscience(R) Mitochondrial Membrane Potential Assay Kit (with JC-1) is developed to identify apoptotic with JC-1 reagent by changing of mitochondrial membrane potential which occur in early apoptotis. This kit provides Carbonyl cyanide m-chlorophenylhydrazone (CCCP)[E-CK-A301C] to induce the decrease of...

This kit detects caspase 8 activity.