Anti-phospho-Platelet Derived Growth Factor Receptor alpha (Tyr742) (PDGFRa)

Platelet-derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185kD which undergoes homo- or heterodimerization into complexes of a and b subunits upon ligand binding, depending on the isoform of PDGF (PDGF-AA, -BB or -AB) that binds. The phosphorylation of tyrosine residues in the ligand-activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras ? Raf ? ERK1&2 pathway. Tyrosine 742 in PDGFRa is a binding site for PI3-kinase. Applications: Suitable for use in Western blotting applications. Other applications not tested. Recommended Dilution: Western Blot: 0.35-1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: NIH3T3 cells +/- PDGF, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.