Anti-phospho-Catenin, beta (Ser45)

Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3beta may destabilize beta-catenin by phosphorylating it at serines 33, 37 and threonine 41 (4). Serine 45 in beta-catenin is phosphorylated by CK1. This likely serves as a priming site for subsequent phosphorylation by GSK-3 (5-7). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 (Mr 92kD), Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.