Anti-Histone H4K5Ac Monoclonal Antibody (RM199)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin. Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of posttranslational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Acetylation of histone H4 at lysine 5 (H4K5Ac) is enriched in promoter regions and coding sequences in mouse hippocampus and is associated with active chromatin. Hippocampal levels of H4K5Ac increase following contextual fear conditioning in mice. Levels of H4K5Ac are elevated in postmortem brain tissue isolated from fetuses with spina bifida compared with healthy controls. Global levels of H4K5Ac are decreased in blast cells from patients with acute myeloid leukemia compared with healthy individuals, and low levels of H4K5Ac are associated with poor prognosis in these patients. Cayman's Histone H4K5Ac Monoclonal Antibody (RM199) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Acetylated Histone H4 Lysine 5. Immunogen: Peptide corresponding to H4K5Ac. Clone Designation: RM199. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H4K5Ac, (-) Unmodified H4K5, (-) H4K8Ac, (-) H4K12Ac, (-) H4K16Ac, (-) H4K20Ac, (-) H4K31Ac, (-) H4K91Ac.