Anti-TPH2 / Tryptophan hydroxylase 2, clone 31T01

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. TPH2 antibody detects Tryptophan hydroxylase 2, encoded by the TPH2 gene. Tryptophan hydroxylase 2 is the rate limiting enzyme in the biosynthesis of serotonin in the brain. By catalyzing the hydroxylation of tryptophan to 5 hydroxytryptophan, which is then decarboxylated to serotonin, this enzyme plays an essential role in neurotransmission, mood regulation, and circadian rhythms. TPH2 antibody provides researchers with a vital tool to study serotonin metabolism, neuronal signaling, and psychiatric disorders.Tryptophan hydroxylase 2 is distinct from TPH1, which performs the same enzymatic reaction but is expressed primarily in peripheral tissues. TPH2 is enriched in serotonergic neurons of the raphe nuclei in the brainstem. Research with TPH2 antibody has revealed that disruption of this enzyme reduces central serotonin production, leading to alterations in behavior, mood, and stress responses. This central role has made TPH2 a focus of studies in depression, anxiety, and other mood disorders.Mutations in TPH2 have been associated with psychiatric disease and developmental disorders. Genetic variants that reduce enzyme activity have been linked to susceptibility to depression, bipolar disorder, and autism spectrum disorders. Studies using TPH2 antibody in postmortem brain tissue have confirmed altered expression in individuals with psychiatric conditions, supporting its clinical relevance. Animal models with disrupted TPH2 expression also display behavioral phenotypes, reinforcing the causal role of this enzyme in serotonin dependent pathways.Beyond psychiatry, TPH2 is relevant in circadian biology, stress physiology, and drug response. Serotonin produced by TPH2 serves as a precursor for melatonin synthesis in the pineal gland, linking it to circadian regulation. Research with TPH2 antibody has shown that stress hormones modulate its expression, suggesting integration of environmental cues with neurotransmitter metabolism. Because many antidepressants and antipsychotics target serotonin pathways, TPH2 remains an important pharmacological research target.TPH2 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting measures enzyme levels in brain lysates, immunohistochemistry demonstrates expression in raphe nuclei and other serotonergic structures, and immunofluorescence allows visualization of neuronal localization. These methods make TPH2 antibody indispensable in neuroscience research.By providing validated TPH2 antibody reagents, NSJ Bioreagents supports research into neurotransmitter metabolism, brain development, and psychiatric disease. Detection of Tryptophan hydroxylase 2 provides researchers with a reliable means to study serotonin pathways in health and disease.